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KMID : 0613820140240030219
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Journal of Life Science
2014 Volume.24 No. 3 p.219 ~ p.226
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Anti-inflammatory Effect of Scutellaria baicalensis Hot Water Extracts Containing Baicalin on Modulation of the Immune System in Raw264.7 Cells
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Park Hye-Jung
Kim Sang-Min
Kwon Hyun-Ju
Lee Hyun-Tae
Kim Byung-Woo
Kim Tae-Hoon
Kim Moon-Moo
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Abstract
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The immune system protects the body from bacterial infection and disease, as well as cancer that develops following the mutation of cells. Aging exerts adverse effects on the immune system, such as chronic inflammation, resulting in rheumatoid arthritis. The purpose of this study was to investigate the anti-inflammatory effectiveness of Scutellaria baicalensis, which contains baicalin. HPLC analysis showed that S. baicalensis hot water extracts (SBWE) contained 42.2 mg/g of baicalin. To evaluate the cytotoxicity of SBWE, an MTT assay was carried out in Raw264.7 cells. No cytotoxicity was observed below 160 μg/ml of SBWE. SBWE at 40 μg/ml reduced the amount of nitric oxide produced by macrophages stimulated with lipopolysaccharide by 40%. In addition, SBWE inhibited phagocytosis stimulated with zymosan. Furthermore, the content of tumor necrosis factor-alpha (TNF-α) produced by the macrophages was decreased in the presence of SBWE in a dose-dependent manner. SBWE also inhibited the production of interleukin-1 beta (IL-1β) in a time course experiment. Moreover, treatment with 20 μg/ml of SBWE remarkably decreased the expression level of cyclooxynase-2. The results provide evidence that SBWE may exert an anti-inflammatory effect through modulation of the immune system.
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KEYWORD
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Interleukin-1 beta, macrophage, phagocytosis, Scutellaria baicalensis, tumor necrosis factor-alpha
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